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trans rescue of a mutant poliovirus RNA polymerase function.

A series of three-nucleotide insertions was engineered into the P2 and P3 coding regions of the T7 expression plasmid pT7(tau)-PV1, which encodes a full-length copy of poliovirus type 1 (Mahoney) cDNA. When RNA derived in vitro from these mutated templates was used to transfect HeLa cells, viable vi...

Ausführliche Beschreibung

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Bibliografische Detailangaben
Veröffentlicht in:J Virol
Hauptverfasser: Charini, W A, Burns, C C, Ehrenfeld, E, Semler, B L
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology (ASM) 1991
Schlagworte:
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC240624/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1850039/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.65.5.2655-2665.1991
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