trans rescue of a mutant poliovirus RNA polymerase function.
A series of three-nucleotide insertions was engineered into the P2 and P3 coding regions of the T7 expression plasmid pT7(tau)-PV1, which encodes a full-length copy of poliovirus type 1 (Mahoney) cDNA. When RNA derived in vitro from these mutated templates was used to transfect HeLa cells, viable vi...
Guardado en:
| Publicado en: | J Virol |
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| Autores principales: | , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
American Society for Microbiology (ASM)
1991
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC240624/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1850039/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.65.5.2655-2665.1991 |
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