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Development of an in vitro mRNA decay system for Escherichia coli: Poly(A) polymerase I is necessary to trigger degradation

Using a novel Escherichia coli in vitro decay system in which polysomes are the source of both enzymes and mRNA, we demonstrate a requirement for poly(A) polymerase I (PAP I) in mRNA turnover. The in vitro decay of two different mRNAs (trxA and lpp) is triggered by the addition of ATP only when poly...

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Hlavní autoři: Ingle, Caroline A., Kushner, Sidney R.
Médium: Artigo
Jazyk:Inglês
Vydáno: The National Academy of Sciences of the USA 1996
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC24022/
https://ncbi.nlm.nih.gov/pubmed/8975250
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