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Development of an in vitro mRNA decay system for Escherichia coli: Poly(A) polymerase I is necessary to trigger degradation

Using a novel Escherichia coli in vitro decay system in which polysomes are the source of both enzymes and mRNA, we demonstrate a requirement for poly(A) polymerase I (PAP I) in mRNA turnover. The in vitro decay of two different mRNAs (trxA and lpp) is triggered by the addition of ATP only when poly...

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發表在:Proc Natl Acad Sci U S A
Principais autores: Ingle, Caroline A., Kushner, Sidney R.
格式: Artigo
語言:Inglês
出版: National Academy of Sciences 1996
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在線閱讀:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC24022/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8975250/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.93.23.12926
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