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The carboxyl terminus of the bacteriophage T4 DNA polymerase is required for holoenzyme complex formation

To further elucidate the mechanism and dynamics of bacteriophage T4 holoenzyme formation, a mutant polymerase in which the last six carboxyl-terminal amino acids are deleted, was constructed, overexpressed, and purified to homogeneity. The mutant polymerase, designated ΔC6 exo(−), is identical to wi...

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Bibliografiske detaljer
Main Authors: Berdis, Anthony J., Soumillion, Patrice, Benkovic, Stephen J.
Format: Artigo
Sprog:Inglês
Udgivet: The National Academy of Sciences of the USA 1996
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC24004/
https://ncbi.nlm.nih.gov/pubmed/8917503
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