The carboxyl terminus of the bacteriophage T4 DNA polymerase is required for holoenzyme complex formation
To further elucidate the mechanism and dynamics of bacteriophage T4 holoenzyme formation, a mutant polymerase in which the last six carboxyl-terminal amino acids are deleted, was constructed, overexpressed, and purified to homogeneity. The mutant polymerase, designated ΔC6 exo(−), is identical to wi...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1996
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC24004/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8917503/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.93.23.12822 |
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