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Isolation and Characterization of Vacuoles from the Ergot Fungus Claviceps purpurea

Protoplasts of Claviceps purpurea were prepared by treatment of mycelium with a lytic mixture of snail gut enzyme and cellulase from Trichoderma viride. Such protoplasts could be efficiently lysed by Triton X-100 treatment at high osmotic pressure without Ca(2+) or Mg(2+), allowing the release of in...

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Hlavní autoři: Keller, Ullrich, Madry, Norbert, Kleinkauf, Horst, Glund, Konrad
Médium: Artigo
Jazyk:Inglês
Vydáno: 1984
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC239753/
https://ncbi.nlm.nih.gov/pubmed/16346510
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