Isolation and Characterization of Vacuoles from the Ergot Fungus Claviceps purpurea
Protoplasts of Claviceps purpurea were prepared by treatment of mycelium with a lytic mixture of snail gut enzyme and cellulase from Trichoderma viride. Such protoplasts could be efficiently lysed by Triton X-100 treatment at high osmotic pressure without Ca(2+) or Mg(2+), allowing the release of in...
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| Publicado no: | Appl Environ Microbiol |
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| Principais autores: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology (ASM)
1984
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC239753/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16346510/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.47.4.710-714.1984 |
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