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Isolation and Characterization of Vacuoles from the Ergot Fungus Claviceps purpurea
Protoplasts of Claviceps purpurea were prepared by treatment of mycelium with a lytic mixture of snail gut enzyme and cellulase from Trichoderma viride. Such protoplasts could be efficiently lysed by Triton X-100 treatment at high osmotic pressure without Ca(2+) or Mg(2+), allowing the release of in...
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| Main Authors: | , , , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
1984
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC239753/ https://ncbi.nlm.nih.gov/pubmed/16346510 |
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