Stimulation of Zero-trans Rates of Lactose and Maltose Uptake into Yeasts by Preincubation with Hexose To Increase the Adenylate Energy Charge

Initial rates of sugar uptake (zero-trans rates) are often measured by incubating yeast cells with radiolabeled sugars for 5 to 30 s and determining the radioactivity entering the cells. The yeast cells used are usually harvested from growth medium, washed, suspended in nutrient-free buffer, and sto...

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Autors principals: Guimarães, Pedro M. R., Multanen, Jyri-Pekka, Domingues, Lucília, Teixeira, José A., Londesborough, John
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology (ASM) 2008
Matèries:
Physiology and Biotechnology
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2394921/
https://ncbi.nlm.nih.gov/pubmed/18378647
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00188-08
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