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Generation and Characterization of Tn5 Insertion Mutations in Pseudomonas syringae pv. tomato

Tn5-induced insertion mutations were generated in the Pseudomonas syringae pv. tomato genome by mating this plant pathogen with an Escherichia coli strain carrying the suicide plasmid vector for Tn5, pGS9. Km(r) transconjugants occurred at frequencies ranging from 2 × 10(−7) to 9 × 10(−6); approxima...

Ausführliche Beschreibung

Gespeichert in:
Bibliografische Detailangaben
Veröffentlicht in:Appl Environ Microbiol
1. Verfasser: Cuppels, Diane A.
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology (ASM) 1986
Schlagworte:
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC238867/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16346988/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.51.2.323-327.1986
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