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Generation and Characterization of Tn5 Insertion Mutations in Pseudomonas syringae pv. tomato

Tn5-induced insertion mutations were generated in the Pseudomonas syringae pv. tomato genome by mating this plant pathogen with an Escherichia coli strain carrying the suicide plasmid vector for Tn5, pGS9. Km(r) transconjugants occurred at frequencies ranging from 2 × 10(−7) to 9 × 10(−6); approxima...

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Bibliografiske detaljer
Hovedforfatter: Cuppels, Diane A.
Format: Artigo
Sprog:Inglês
Udgivet: 1986
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC238867/
https://ncbi.nlm.nih.gov/pubmed/16346988
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