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Generation and Characterization of Tn5 Insertion Mutations in Pseudomonas syringae pv. tomato

Tn5-induced insertion mutations were generated in the Pseudomonas syringae pv. tomato genome by mating this plant pathogen with an Escherichia coli strain carrying the suicide plasmid vector for Tn5, pGS9. Km(r) transconjugants occurred at frequencies ranging from 2 × 10(−7) to 9 × 10(−6); approxima...

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主要作者: Cuppels, Diane A.
格式: Artigo
語言:Inglês
出版: 1986
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC238867/
https://ncbi.nlm.nih.gov/pubmed/16346988
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