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Construction and Use of New Cloning Vectors for the Rapid Isolation of Recombinant Proteins from Escherichia coli
We describe the construction and use of two sets of vectors for the over-expression and purification of protein from E. coli. The set of pTEV plasmids (pTEV3, 4, 5) directs the synthesis of a recombinant protein with a N-terminal hexahistidine (His(6)) tag that is removable by the tobacco etch virus...
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| Главные авторы: | , , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
2008
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2386272/ https://ncbi.nlm.nih.gov/pubmed/18295882 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.plasmid.2008.01.001 |
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