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New high-cloning-efficiency vectors for complementation studies and recombinant protein overproduction in Escherichia coli and Salmonella enterica

Galloway et al. recently described a method to alter vectors to include Type IIS restriction enzymes for high efficiency cloning. Utilizing this method, the multiple cloning sites of complementation and overexpression vectors commonly used in our laboratory were altered to contain recognition sequen...

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Detalhes bibliográficos
Publicado no:Plasmid
Main Authors: VanDrisse, C.M., Escalante-Semerena, J.C.
Formato: Artigo
Idioma:Inglês
Publicado em: 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5126758/
https://ncbi.nlm.nih.gov/pubmed/27234933
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.plasmid.2016.05.001
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