Amplification and sequence analysis of DNA flanking integrated proviruses by a simple two-step polymerase chain reaction method.
We describe a two-step polymerase chain reaction method that can be used for the amplification of cellular DNA sequences adjacent to an integrated retroviral provirus. The technique involves a partly degenerate, arbitrary primer that will hybridize in the provirus-flanking cellular DNA. By using thi...
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| 出版年: | J Virol |
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| 主要な著者: | , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
American Society for Microbiology (ASM)
1993
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC238173/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8230434/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.67.12.7118-7124.1993 |
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