Amplification and sequence analysis of DNA flanking integrated proviruses by a simple two-step polymerase chain reaction method.

We describe a two-step polymerase chain reaction method that can be used for the amplification of cellular DNA sequences adjacent to an integrated retroviral provirus. The technique involves a partly degenerate, arbitrary primer that will hybridize in the provirus-flanking cellular DNA. By using thi...

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Bibliographic Details

Published in:	J Virol
Main Authors:	Sørensen, A B, Duch, M, Jørgensen, P, Pedersen, F S
Format:	Artigo
Language:	Inglês
Published:	American Society for Microbiology (ASM) 1993
Subjects:	Research Article
Online Access:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC238173/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8230434/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.67.12.7118-7124.1993
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