Amplification and sequence analysis of DNA flanking integrated proviruses by a simple two-step polymerase chain reaction method.

We describe a two-step polymerase chain reaction method that can be used for the amplification of cellular DNA sequences adjacent to an integrated retroviral provirus. The technique involves a partly degenerate, arbitrary primer that will hybridize in the provirus-flanking cellular DNA. By using thi...

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Sparad:
Bibliografiska uppgifter
Huvudupphovsmän: Sørensen, A B, Duch, M, Jørgensen, P, Pedersen, F S
Materialtyp: Artigo
Språk:Inglês
Publicerad: 1993
Ämnen:
Research Article
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC238173/
https://ncbi.nlm.nih.gov/pubmed/8230434
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