Amplification and sequence analysis of DNA flanking integrated proviruses by a simple two-step polymerase chain reaction method.

We describe a two-step polymerase chain reaction method that can be used for the amplification of cellular DNA sequences adjacent to an integrated retroviral provirus. The technique involves a partly degenerate, arbitrary primer that will hybridize in the provirus-flanking cellular DNA. By using thi...

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Detalhes bibliográficos

Publicado no:	J Virol
Principais autores:	Sørensen, A B, Duch, M, Jørgensen, P, Pedersen, F S
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1993
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC238173/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8230434/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.67.12.7118-7124.1993
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