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Coupled translation and replication of poliovirus RNA in vitro: synthesis of functional 3D polymerase and infectious virus.

Poliovirus RNA polymerase and infectious virus particles were synthesized by translation of virion RNA in vitro in HeLa S10 extracts. The in vitro translation reactions were optimized for the synthesis of the viral proteins found in infected cells and in particular the synthesis of the viral polymer...

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Détails bibliographiques
Publié dans:J Virol
Auteurs principaux: Barton, D J, Flanegan, J B
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology (ASM) 1993
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC237436/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8380467/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.67.2.822-831.1993
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