Characterization and crystallization of a recombinant IgE Fab fragment in complex with the bovine β-lactoglobulin allergen

A D1 Fab fragment containing the allergen-binding variable domains of the IgE antibody was characterized by ESI FT–ICR mass spectrometry and crystallized with bovine β-lactoglobulin (BLG) using the hanging-drop vapour-diffusion method at 293 K. X-ray data suitable for structure determination were co...

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Main Authors: Niemi, Merja, Jänis, Janne, Jylhä, Sirpa, Kallio, Johanna M., Hakulinen, Nina, Laukkanen, Marja-Leena, Takkinen, Kristiina, Rouvinen, Juha
Format: Artigo
Jezik:Inglês
Izdano: International Union of Crystallography 2007
Teme:
Crystallization Communications
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2373997/
https://ncbi.nlm.nih.gov/pubmed/18097096
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S174430910706160X
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