Changing the net charge from negative to positive makes ribonuclease Sa cytotoxic

Ribonuclease Sa (pI = 3.5) from Streptomyces aureofaciens and its 3K (D1K, D17K, E41K) (pI = 6.4) and 5K (3K + D25K, E74K) (pI = 10.2) mutants were tested for cytotoxicity. The 5K mutant was cytotoxic to normal and v-ras-transformed NIH3T3 mouse fibroblasts, but RNase Sa and 3K were not. The structu...

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Bibliographic Details
Main Authors: Ilinskaya, Olga N., Dreyer, Florian, Mitkevich, Vladimir A., Shaw, Kevin L., Pace, C. Nick, Makarov, Alexander A.
Format: Artigo
Language:Inglês
Published: Cold Spring Harbor Laboratory Press 2002
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2373699/
https://ncbi.nlm.nih.gov/pubmed/12237473
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