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Detection of four oxidation sites in viral prolyl-4-hydroxylase by top-down mass spectrometry

Oxidative inactivation is a common problem for enzymatic reactions that proceed via iron oxo intermediates. In an investigation of the inactivation of a viral prolyl-4-hydroxylase (26 kD), electrospray mass spectrometry (MS) directly shows the degree of oxidation under varying experimental condition...

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Bibliografiske detaljer
Main Authors: Ge, Ying, Lawhorn, Brian G., ElNaggar, Mariam, Sze, Siu Kwan, Begley, Tadhg P., McLafferty, Fred W.
Format: Artigo
Sprog:Inglês
Udgivet: Cold Spring Harbor Laboratory Press 2003
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2366917/
https://ncbi.nlm.nih.gov/pubmed/14500890
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