Panhandle PCR strategy to amplify MLL genomic breakpoints in treatment-related leukemias

Panhandle PCR amplifies genomic DNA with known 5′ and unknown 3′ sequences from a template with an intrastrand loop schematically shaped like a pan with a handle. We used panhandle PCR to clone MLL genomic breakpoints in two pediatric treatment-related leukemias. The karyotype in a case of treatment...

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Main Authors: Megonigal, Maureen D., Rappaport, Eric F., Jones, Douglas H., Kim, Caroline S., Nowell, Peter C., Lange, Beverly J., Felix, Carolyn A.
Format: Artigo
Sprog:Inglês
Udgivet: The National Academy of Sciences of the USA 1997
Fag:
Biological Sciences
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC23546/
https://ncbi.nlm.nih.gov/pubmed/9326653
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