Direct isolation of human transcribed sequences from yeast artificial chromosomes through the application of RNA fingerprinting
The identification of cDNA clones from genomic regions known to contain human genes is usually the rate-limiting factor in positional cloning strategies. We demonstrate here that human genes present on yeast artificial chromosomes (YACs) are transcribed in yeast host cells. We have used the arbitrar...
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| Publicado en: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
National Academy of Sciences
1997
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC23370/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9294218/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.94.19.10373 |
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