Direct isolation of human transcribed sequences from yeast artificial chromosomes through the application of RNA fingerprinting

The identification of cDNA clones from genomic regions known to contain human genes is usually the rate-limiting factor in positional cloning strategies. We demonstrate here that human genes present on yeast artificial chromosomes (YACs) are transcribed in yeast host cells. We have used the arbitrar...

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Pubblicato in:	Proc Natl Acad Sci U S A
Autori principali:	Still, Ivan H., Vince, Pauline, Cowell, John K.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	National Academy of Sciences 1997
Soggetti:	Biological Sciences
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC23370/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9294218/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.94.19.10373
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