Direct isolation of human transcribed sequences from yeast artificial chromosomes through the application of RNA fingerprinting
The identification of cDNA clones from genomic regions known to contain human genes is usually the rate-limiting factor in positional cloning strategies. We demonstrate here that human genes present on yeast artificial chromosomes (YACs) are transcribed in yeast host cells. We have used the arbitrar...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1997
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC23370/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9294218/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.94.19.10373 |
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