Overexpression, purification, crystallization and preliminary structural studies of catabolic ornithine transcarbamylase from Lactobacillus hilgardii

The catabolic ornithine transcarbamylase (cOTC; EC 2.1.3.3) from the lactic acid bacteria Lactobacillus hilgardii is a key protein involved in the degradation of arginine during malolactic fermentation. cOTC containing an N-terminal His(6) tag has been overexpressed in Escherichia coli, purified and...

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Bibliographic Details
Main Authors: de las Rivas, Blanca, Rodríguez, Héctor, Angulo, Iván, Muñoz, Rosario, Mancheño, José M.
Format: Artigo
Language:Inglês
Published: International Union of Crystallography 2007
Subjects:
Crystallization Communications
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2335135/
https://ncbi.nlm.nih.gov/pubmed/17620711
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309107025195
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