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Overexpression, purification, crystallization and preliminary structural studies of p-coumaric acid decarboxylase from Lactobacillus plantarum

The substrate-inducible p-coumaric acid decarboxylase (PDC) from Lactobacillus plantarum has been overexpressed in Escherichia coli, purified and confirmed to possess decarboxylase activity. The recombinant His(6)-tagged enzyme was crystallized using the hanging-drop vapour-diffusion method from a s...

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Detalhes bibliográficos
Main Authors: Rodríguez, Héctor, de las Rivas, Blanca, Muñoz, Rosario, Mancheño, José M.
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2330202/
https://ncbi.nlm.nih.gov/pubmed/17401200
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309107008846
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