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Structure of the T109S mutant of Escherichia coli dihydroorotase complexed with the inhibitor 5-­fluoroorotate: catalytic activity is reflected by the crystal form

Crystals of a single-point mutant (T109S) of Escherichia coli dihydroorotase (DHOase) with diminished activity grown in the presence of l-dihydroorotate (l-DHO) are tetragonal, with a monomer in the asymmetric unit. These crystals are extremely unstable and disintegrate shortly after formation, whic...

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Autors principals: Lee, Mihwa, Maher, Megan J., Guss, J. Mitchell
Format: Artigo
Idioma:Inglês
Publicat: International Union of Crystallography 2007
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2330171/
https://ncbi.nlm.nih.gov/pubmed/17329804
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309107004009
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