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In Vivo Assay for Low-Activity Mutant Forms of Escherichia coli Ribonucleotide Reductase

Ribonucleotide reductase (RNR) catalyzes the essential production of deoxyribonucleotides in all living cells. In this study we have established a sensitive in vivo assay to study the activity of RNR in aerobic Escherichia coli cells. The method is based on the complementation of a chromosomally enc...

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Détails bibliographiques
Auteurs principaux: Ekberg, Monica, Birgander, Pernilla, Sjöberg, Britt-Marie
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology 2003
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC142847/
https://ncbi.nlm.nih.gov/pubmed/12562785
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.185.4.1167-1173.2003
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