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Purification, crystallization and preliminary crystallographic analysis of the putative thiamine-biosynthesis protein PH1313 from Pyrococcus horikoshii OT3

The putative thiamine-biosynthesis protein PH1313 from Pyrococcus horikoshii OT3 has been overexpressed and purified. Crystallization was performed by the oil-microbatch method using 28%(v/v) 2-methyl-2,4-pentanediol as a precipitant at 291 K. A native X-ray diffraction data set at 1.9 Å resolution...

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Bibliografiske detaljer
Main Authors: Sugahara, Michihiro, Murai, Satoko, Sugahara, Mitsuaki, Kunishima, Naoki
Format: Artigo
Sprog:Inglês
Udgivet: International Union of Crystallography 2006
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2330111/
https://ncbi.nlm.nih.gov/pubmed/17183176
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309106054509
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