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Purification, crystallization and preliminary X-ray analysis of the BseCI DNA methyltransferase from Bacillus stearothermophilus in complex with its cognate DNA

The DNA methyltransferase M.BseCI from Bacillus stearothermophilus (EC 2.1.1.72), a 579-amino-acid enzyme, methylates the N6 atom of the 3′ adenine in the sequence 5′-ATCGAT-3′. M.BseCI was crystallized in complex with its cognate DNA. The crystals were found to belong to the hexagonal space group P...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Kapetaniou, Evangelia G., Kotsifaki, Dina, Providaki, Mary, Rina, Maria, Bouriotis, Vassilis, Kokkinidis, Michael
Format: Artigo
Sprache:Inglês
Veröffentlicht: International Union of Crystallography 2006
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2330102/
https://ncbi.nlm.nih.gov/pubmed/17183163
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309106051530
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