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Purification, crystallization and preliminary X-ray diffraction analysis of a variant of the ColE1 Rop protein

Rop is the paradigm of a canonical four-α-helical bundle. Its loop region has attracted considerable interest because a single alanine-to-proline substitution (A31P) in the loop is sufficient to change the topology of this small protein. In order to further analyse the loop region as a possible fold...

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Detalhes bibliográficos
Main Authors: Ambrazi, Maria, Fellas, George, Kapetaniou, Evangelia G., Kotsifaki, Dina, Providaki, Mary, Kokkinidis, Michael
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2008
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2376389/
https://ncbi.nlm.nih.gov/pubmed/18453719
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309108011342
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