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Improved production of human type II procollagen in the yeast Pichia pastoris in shake flasks by a wireless-controlled fed-batch system

BACKGROUND: Here we describe a new technical solution for optimization of Pichia pastoris shake flask cultures with the example of production of stable human type II collagen. Production of recombinant proteins in P. pastoris is usually performed by controlling gene expression with the strong AOX1 p...

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Dettagli Bibliografici
Autori principali: Ruottinen, Maria, Bollok, Monika, Kögler, Martin, Neubauer, Antje, Krause, Mirja, Hämäläinen, Eija-Riitta, Myllyharju, Johanna, Vasala, Antti, Neubauer, Peter
Natura: Artigo
Lingua:Inglês
Pubblicazione: BioMed Central 2008
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2315644/
https://ncbi.nlm.nih.gov/pubmed/18371201
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-8-33
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