Antifoam addition to shake flask cultures of recombinant Pichia pastoris increases yield

BACKGROUND: Pichia pastoris is a widely-used host for recombinant protein production. Initial screening for both suitable clones and optimum culture conditions is typically carried out in multi-well plates. This is followed by up-scaling either to shake-flasks or continuously stirred tank bioreactor...

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Hlavní autoři: Routledge, Sarah J, Hewitt, Christopher J, Bora, Nagamani, Bill, Roslyn M
Médium: Artigo
Jazyk:Inglês
Vydáno: BioMed Central 2011
Témata:
Research
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3072307/
https://ncbi.nlm.nih.gov/pubmed/21426555
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2859-10-17
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