A novel fed-batch based cultivation method provides high cell-density and improves yield of soluble recombinant proteins in shaken cultures

BACKGROUND: Cultivations for recombinant protein production in shake flasks should provide high cell densities, high protein productivity per cell and good protein quality. The methods described in laboratory handbooks often fail to reach these goals due to oxygen depletion, lack of pH control and t...

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Detalhes bibliográficos
Main Authors: Krause, Mirja, Ukkonen, Kaisa, Haataja, Tatu, Ruottinen, Maria, Glumoff, Tuomo, Neubauer, Antje, Neubauer, Peter, Vasala, Antti
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2010
Assuntos:
Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2841585/
https://ncbi.nlm.nih.gov/pubmed/20167131
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2859-9-11
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