A novel fed-batch based cultivation method provides high cell-density and improves yield of soluble recombinant proteins in shaken cultures

BACKGROUND: Cultivations for recombinant protein production in shake flasks should provide high cell densities, high protein productivity per cell and good protein quality. The methods described in laboratory handbooks often fail to reach these goals due to oxygen depletion, lack of pH control and t...

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Hlavní autoři: Krause, Mirja, Ukkonen, Kaisa, Haataja, Tatu, Ruottinen, Maria, Glumoff, Tuomo, Neubauer, Antje, Neubauer, Peter, Vasala, Antti
Médium: Artigo
Jazyk:Inglês
Vydáno: BioMed Central 2010
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Research
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2841585/
https://ncbi.nlm.nih.gov/pubmed/20167131
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2859-9-11
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