Rapid detection of Pneumocystis carinii in bronchoalveolar lavage specimens from human immunodeficiency virus-infected patients: use of a simple DNA extraction procedure and nested PCR.

We report on the development of a rapid nested PCR protocol for the detection of Pneumocystis carinii DNA in bronchoalveolar lavage (BAL) specimens in which the protocol included the use of a commercially available DNA extraction kit (GeneReleaser). GeneReleaser enabled us to obtain amplification-re...

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Bibliografiske detaljer

Udgivet i:	J Clin Microbiol
Principais autores:	Rabodonirina, M, Raffenot, D, Cotte, L, Boibieux, A, Mayençon, M, Bayle, G, Persat, F, Rabatel, F, Trepo, C, Peyramond, D, Piens, M A
Format:	Artigo
Sprog:	Inglês
Udgivet:	American Society for Microbiology (ASM) 1997
Fag:	Research Article
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC230054/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9350726/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.35.11.2748-2751.1997
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