Rapid detection of Pneumocystis carinii in bronchoalveolar lavage specimens from human immunodeficiency virus-infected patients: use of a simple DNA extraction procedure and nested PCR.

We report on the development of a rapid nested PCR protocol for the detection of Pneumocystis carinii DNA in bronchoalveolar lavage (BAL) specimens in which the protocol included the use of a commercially available DNA extraction kit (GeneReleaser). GeneReleaser enabled us to obtain amplification-re...

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Detalhes bibliográficos
Main Authors: Rabodonirina, M, Raffenot, D, Cotte, L, Boibieux, A, Mayençon, M, Bayle, G, Persat, F, Rabatel, F, Trepo, C, Peyramond, D, Piens, M A
Formato: Artigo
Idioma:Inglês
Publicado em: 1997
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC230054/
https://ncbi.nlm.nih.gov/pubmed/9350726
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