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Rapid detection of Pneumocystis carinii in bronchoalveolar lavage specimens from human immunodeficiency virus-infected patients: use of a simple DNA extraction procedure and nested PCR.

We report on the development of a rapid nested PCR protocol for the detection of Pneumocystis carinii DNA in bronchoalveolar lavage (BAL) specimens in which the protocol included the use of a commercially available DNA extraction kit (GeneReleaser). GeneReleaser enabled us to obtain amplification-re...

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Bibliografische gegevens
Gepubliceerd in:J Clin Microbiol
Hoofdauteurs: Rabodonirina, M, Raffenot, D, Cotte, L, Boibieux, A, Mayençon, M, Bayle, G, Persat, F, Rabatel, F, Trepo, C, Peyramond, D, Piens, M A
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: American Society for Microbiology (ASM) 1997
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC230054/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9350726/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.35.11.2748-2751.1997
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