A single-tube nested PCR for Pneumocystis carinii f. sp. hominis.
A single-tube nested PCR which amplifies the internal transcribed spacer (ITS) regions of the rRNA genes of human Pneumocystis carinii was developed. The outer primers for the first PCR, which anneal to the 18S and the 26S rRNA genes of P. carinii, were made to have a midpoint temperature (Tm) of 74...
Enregistré dans:
| Publié dans: | J Clin Microbiol |
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| Auteurs principaux: | , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
American Society for Microbiology (ASM)
1997
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC229797/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9163492/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.35.6.1597-1599.1997 |
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