A single-tube nested PCR for Pneumocystis carinii f. sp. hominis.

A single-tube nested PCR which amplifies the internal transcribed spacer (ITS) regions of the rRNA genes of human Pneumocystis carinii was developed. The outer primers for the first PCR, which anneal to the 18S and the 26S rRNA genes of P. carinii, were made to have a midpoint temperature (Tm) of 74...

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Bibliografiske detaljer

Udgivet i:	J Clin Microbiol
Principais autores:	Tang, X, Bartlett, M S, Smith, J W, Lee, C H
Format:	Artigo
Sprog:	Inglês
Udgivet:	American Society for Microbiology (ASM) 1997
Fag:	Research Article
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC229797/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9163492/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.35.6.1597-1599.1997
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