A single-tube nested PCR for Pneumocystis carinii f. sp. hominis.

A single-tube nested PCR which amplifies the internal transcribed spacer (ITS) regions of the rRNA genes of human Pneumocystis carinii was developed. The outer primers for the first PCR, which anneal to the 18S and the 26S rRNA genes of P. carinii, were made to have a midpoint temperature (Tm) of 74...

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Vydáno v:	J Clin Microbiol
Hlavní autoři:	Tang, X, Bartlett, M S, Smith, J W, Lee, C H
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	American Society for Microbiology (ASM) 1997
Témata:	Research Article
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC229797/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9163492/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.35.6.1597-1599.1997
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