Detection of Cryptosporidium parvum DNA in formed human feces by a sensitive PCR-based assay including uracil-N-glycosylase inactivation.

We developed a PCR-based method that can be used to identify Cryptosporidium parvum in human feces. Fecal oocysts were concentrated by centrifugation on a sodium chloride gradient and filtration on a nitrocellulose filter prior to DNA extraction and PCR amplification of a 452-bp C. parvum-specific D...

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Detalhes bibliográficos

Publicado no:	J Clin Microbiol
Principais autores:	Gobet, P, Buisson, J C, Vagner, O, Naciri, M, Grappin, M, Comparot, S, Harly, G, Aubert, D, Varga, I, Camerlynck, P, Bonnin, A
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1997
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC229549/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8968918/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.35.1.254-256.1997
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