Detection of Cryptosporidium parvum DNA in formed human feces by a sensitive PCR-based assay including uracil-N-glycosylase inactivation.

We developed a PCR-based method that can be used to identify Cryptosporidium parvum in human feces. Fecal oocysts were concentrated by centrifugation on a sodium chloride gradient and filtration on a nitrocellulose filter prior to DNA extraction and PCR amplification of a 452-bp C. parvum-specific D...

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Bibliographic Details
Main Authors: Gobet, P, Buisson, J C, Vagner, O, Naciri, M, Grappin, M, Comparot, S, Harly, G, Aubert, D, Varga, I, Camerlynck, P, Bonnin, A
Format: Artigo
Language:Inglês
Published: 1997
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC229549/
https://ncbi.nlm.nih.gov/pubmed/8968918
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