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Automated Template Quantification for DNA Sequencing Facilities

The quantification of plasmid DNA by the PicoGreen dye binding assay has been automated, and the effect of quantification of user-submitted templates on DNA sequence quality in a core laboratory has been assessed. The protocol pipets, mixes and reads standards, blanks and up to 88 unknowns, generate...

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Detalles Bibliográficos
Main Authors: Ivanetich, Kathryn M., Yan, Wilson, Wunderlich, Kathleen M., Weston, Jennifer, Walkup, Ward G., Simeon, Christian
Formato: Artigo
Idioma:Inglês
Publicado: The Association of Biomolecular Resource Facilities 2005
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2291726/
https://ncbi.nlm.nih.gov/pubmed/16461949
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