Differences in aggregation properties of three site-specific mutants of recombinant human stefin B

We describe expression, purification, and characterization of three site-specific mutants of recombinant human stefin B: H75W, P36G, and P79S. The far- and near-UV CD spectra have shown that they have similar secondary and tertiary structures to the parent protein. The elution on gel-filtration sugg...

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Detaylı Bibliyografya
Asıl Yazarlar: Kenig, Manca, Berbić, Selma, Krijes̆torac, Aida, Kroon-Z̆itko, Louise, Tus̆ek, Magda, Pompe-Novak, Marus̆a, Z̆erovnik, Eva
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Cold Spring Harbor Laboratory Press 2004
Konular:
Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC2286520/
https://ncbi.nlm.nih.gov/pubmed/14691222
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.03270904
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