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Differences in aggregation properties of three site-specific mutants of recombinant human stefin B
We describe expression, purification, and characterization of three site-specific mutants of recombinant human stefin B: H75W, P36G, and P79S. The far- and near-UV CD spectra have shown that they have similar secondary and tertiary structures to the parent protein. The elution on gel-filtration sugg...
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| Auteurs principaux: | , , , , , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Cold Spring Harbor Laboratory Press
2004
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2286520/ https://ncbi.nlm.nih.gov/pubmed/14691222 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.03270904 |
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