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Analysis of subsecond protein dynamics by amide hydrogen exchange and mass spectrometry using a quenched-flow setup
Amide hydrogen exchange (HX) in combination with mass spectrometry (MS) is a powerful tool to analyze the folding and dynamics of proteins. In the traditional methodology the exchange time is controlled by manual pipetting, thereby limiting the time resolution to several seconds. Some conformational...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Cold Spring Harbor Laboratory Press
2005
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2279298/ https://ncbi.nlm.nih.gov/pubmed/15689511 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.041098305 |
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