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High-throughput biochemical analysis of in vivo location data reveals novel distinct classes of POU5F1(Oct4)/DNA complexes

The transcription factor POU5F1 is a key regulator of embryonic stem (ES) cell pluripotency and a known oncoprotein. We have developed a novel high-throughput binding assay called MEGAshift (microarray evaluation of genomic aptamers by shift) that we use to pinpoint the exact location, affinity, and...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Tantin, Dean, Gemberling, Matthew, Callister, Catherine, Fairbrother, William
Format: Artigo
Sprache:Inglês
Veröffentlicht: Cold Spring Harbor Laboratory Press 2008
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2279250/
https://ncbi.nlm.nih.gov/pubmed/18212089
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.072942.107
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