Simultaneous identification of strains of Escherichia coli serotype O157:H7 and their Shiga-like toxin type by mismatch amplification mutation assay-multiplex PCR.

Mismatch amplification mutation assay primers, specific for a unique base substitution in uidA of Escherichia coli O157:H7, was coupled with primers for the Shiga-like toxin I (SLT-I) and SLT-II genes in a multiplex PCR assay. Analysis of 108 bacteria showed that all Escherichia coli serotype O157:H...

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Detalhes bibliográficos
Main Authors: Cebula, T A, Payne, W L, Feng, P
Formato: Artigo
Idioma:Inglês
Publicado em: 1995
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Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC227922/
https://ncbi.nlm.nih.gov/pubmed/7535315
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